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8:32 p.m.

Mrs. F. my Dear,
Have a look at the gel. Are you happy with it ?
I promise I did not manipulate anything on it: no make-up, no hair dye, mascara, no plastic surgery. It is real, and therefore it is beautiful (just like you are).
M.
PS: RNA I”ll tell you in a moment. Stay tuned !!!!

Mrs. F.s-pcr-gel-late-night-15092010

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8:44 p.m.

thank you for the gel!!! very kind of you. still the prop1 exon2 JF1 show a lot of bands.and is a bit weak. and the first three bands being prop1 exon1, i see two bands for each sample? or is it the primer dimers? and on the last samples on the first row (being gpx3 exon1), i see many bands or is it good because of the thick band?
on the second row gpx3 exon3- some extra bands as well. or is it okay? so i can use this for the sequencing tomorrow?

i am looking forward to hear about the rna. this is exciting i have to admit!!!

good luck! i will try to look at my thesis now. so i do something useful as well.

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9:48 p.m.

Mrs. F., my Dear

So happy to have brain RNA now (one JF1 one FVB, each high concentration).
So sad to sit here alone. Nobody to celebrate with. Its boring alone, even the results are o.k.
brain-rna

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9:54 p.m.

michael!!!! yey!!! looks really good! im happy too! you did a great job!! so are we going to kill more mice? and then do the extracation of RNA your way?

thank you michael, for putting so much time and energy for this project.

you should go home and get some rest.

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10:06 p.m.
Mrs. F. dear,

On 15.09.2010 21:54, Mrs. F. F wrote:
> michael!!!! yey!!! looks really good! im happy too! you did a great job!! so are we going to kill more mice?

I already have thyroids from two mice each strain. I start now with the extraction. Hope of equally good results. Lets see.

> and then do the extracation of RNA your way?

Its not my way. Its the classical way (it stinks, it is more time consuming, but you see at the end works more robust).

>
> thank you michael, for putting so much time and energy for this project.

Its our project. Don”t forget what you put in it: 5 month lonely life , the people you love so far away. Nobody here to kiss you before going to bed.

>
> you should go home and get some rest.

Where is this, home ?

Take Care, my dear (and don”t you walk around on empty streets in the darkness any more).
Mrs. F., I got mad if I think that something happens to you. You remember you talked about Sweden and
that raping happens there more often. Thats in fact one of the main reasons I always got a shock when I hear or read that you are going back.

Michael

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10:56 p.m.

> I already have thyroids from two mice each strain. I start now with the
> extraction. Hope of equally good results. Lets see.
michael, we can continue tomorrow?
you are doing too much. i also want to be done with the labwork this week but dont want you to do all this and stay for so long in the lab.

> Where is this, home ?
your home, where your family is and your bed:)

> Take Care, my dear (and don”t you walk around on empty streets in the
> darkness any more). Mrs. F., I got mad if I think that something happens to you. You remember
> you talked about Sweden and that raping happens there more often. Thats in fact one of the main
> reasons I always got a shock when I hear or read that you are going back.

i did not walk in the night,. it was light. i am scared to walk alone at night. and yes, i find munich as a safe place. safer than sweden. i need to walk sometimes, instead of taking bike i walk sometimes, its nice. nice feeling when you walk alone with your own thoughts.

hope u are doing well in lab and that u are going home soon…
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10:51 p.m.
On 15.09.2010 21:54, Mrs. F. F wrote:

> michael!!!! yey!!! looks really good! im happy too! you did a great job!! so are we going to kill more mice?
Who is going to kill mice ? You ? Maybe you first practise this on me. But be aware: You can kill me with your gun, but not the mice. If you hold a gun on me, I wont move away, cause I”ll be struck again by the glance of your eyes. Not so the mice: They can smell the danger and they run away !!

Mrs. F., I got the feeling we can speak open to each other, can”t we ?

TAKE CARE

Michael
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10:58 p.m.
noooo i am not killing anyone.
yes we can talk open.. or how do you mean
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11:04 p.m.
Mrs. F. my Dear,
Just let me start the Reverse Transcription. (I do it in a PCR cycler, can be programmed to do it and after 1 hour cooles down automatically).
Then you have the cDNA tomorrow morning and can start with PCR right away.

On 15.09.2010 22:58, Mrs. F. F wrote:
> noooo i am not killing anyone.
> yes we can talk open.. or how do you mean

I”ll come around and tell you.

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11:09 p.m.
come around? i will sleep now also my flatmate is sleeping. so see you tomorrow. thank you again for the cdna!!!!
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11:24 p.m.

On 15.09.2010 23:09, Mrs. F. F wrotei:
> come around? i will sleep now also my flatmate is sleeping.
I”m not going to wake up your flatmate !!
And you said you are going to work on your thesis the whole night through ?
Did the thesis made you already such tired ?

> so see you tomorrow. thank you again for the cdna!!!!
This, of course was very, very open !!!
And this is what I like you for so much.

Then good night, funny dreams, and at least you can imagine that I kissed you before going to bed.

Take Care

Michael
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0:00 a.m.
Mrs. F. my dear,
Are you really already asleep ? This was a fast thesis writing !!
Hope I haven”t scared you.

Sleep well
Michael
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0:04 a.m.
was trying to sleep. when i saw your email of what you expected of me, to write the thesis, i couldnt sleep. will try to read a bit more now…

good night
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0:11 a.m.

Mrs. F. my Dear, please no no, shame on me, I didn”t ment it.
You do everything right. The night is yours. You work the whole day already. Please stop to spoil the best hours of the day with the thesis now. Sleep, please.
I”m also almost done. Don”t worry, I won”t pass by. Maybe another time, not today. It would be silly to first drive away, just to come back later.

I like you anyhow. I will always do.

Michael
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0:20 a.m.
if you get hungry i have a sandwich in the fridge.
hope u will be done soon so u can go home..

good night

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0:44 a.m.
Mrs. F. my Dear,

Sorry for the assumption that you could kill me. Your last e-mail shows how much you care about me.
I found one sandwich. Hope it is the one that you offered: It is brownish, with sliced boiled eggs on it and some letuce, right.

Please tell me it is the right one, it looks delicious.

Something I hope you are delighted (just for your intellectual satisfaction) is the next RNAs: You see they are all above 1µg and ration around 1.8.

thyroid-brain-rna